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The Kirby Lab Neonatal Perinatal Research Institute

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Harriett Stadt, HTL
Research Assistant

I have been working with the Kirby Lab
since 1987 and we are currently working
on a number of projects. I am a member
of the microsurgery team whose purpose
is to supply group members with embryos
that have had neural crest ablation
performed with a low nitrogen laser.
Karen Waldo and I are looking at cell
death with ToPro in the secondary heart
field of some of these experimental and
sham operated embryos. In Mary Hudson’s
electroporation experiments where she is
altering FgF8 and BMP signaling pathways
we are working on the antibody stain for
FGF8 since identifying normal and abnormal
expression will be important.

Progress has been steady and future success
seems promising. The projects and the people
have added up to 15 years of good work for me.

Recent publications

Kirby ML, Lawson A, Stadt HA, Kumiski DH, Wallis KT, McCraney E, Waldo KL, Schoenwolf G.
2002 Prechordal plate gives rise to the midline endoderm of the foregut,
a longitudinal stripe having characteristics of a ventral axis (submitted)

Yelbuz TM, Waldo KL, Kumiski DH, Stadt HA, Wolfe RR, Leatherbury LL, Kirby ML
2002 Shortened outflow tract leads to altered cardiac looping after neural crest ablation.
Circulation 106, 504-510.

Farrell MJ, Burch JL, Kumiski DH, Stadt HA, Godt RE, Creazzo TL, & Kirby ML
2001. FGF-8 suppresses development of myocardial calcium transients after neural crest ablation.
J. Clin. Invest. 107:1509-1517.

Waldo KL, Zdanowicz M, Burch JL, Kumiski DH, Stadt HA, Godt RE, Creazzo TL, and Kirby ML 1999.
A novel role for cardiac neural crest in heart development.
J Clin Invest 103:1499-1507.

Favorite data If the lateral edges of the anterior intestinal portal are ablated by laser while the ventral foregut is closing, as shown in these images, then heart looping is randomized suggesting that the anterior intestinal portal carries right-left patterning information. The green stripes in B show the dead cells after laser ablation which can also be seen at the arrow in C.